In Vitro ADME

In vitro ADME screens provide critical information for lead optimization and selection of the appropriate species for conduct of toxicological studies. Elucidation of the interaction of a test substance under investigation using human cytochrome(s) P450 (CYP) and other drug-metabolizing enzymes is important for predicting its potential for interaction with other drugs which is of great relevance in the planning of clinical studies.

At present IIRT has capability to conduct the following studies.

Absorption studies

• Cell permeability assays (Caco2 and MDCK cells)
• Parallel Artificial Membrane Permeability Assay (PAMPA)
• Everted Gut Sac Permeability Assay

Drug – Drug Interaction studies

• Cytochrome P450 Inhibition

Metabolic stability, metabolic identification and estimation of reactive metabolites using liver microsomes

• Clearance and half life determination

Physico-chemical Studies

• Solubility studies for NCE – Kinetic and Equilibrium solubility
• Determination of Log P and Log D

Plasma / Whole blood studies

• Plasma Protein Binding – mouse, rat, rabbit, dog and human
• Plasma / Whole blood stability – mouse, rat, rabbit, dog and human

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